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排序方式: 共有262条查询结果,搜索用时 375 毫秒
101.
102.
103.
Anatoly F. Vanin Vasak D. Mikoyan Nikolai M. Rubtsov Lioudmila N. Kubrina 《Nitric oxide》2010,22(3):175-180
It is hypothesized that in cells producing nitric oxide (NO), NO and its endogenous derivatives (low-molecular S-nitrosothiols and dinitrosyl iron complexes (DNIC) with thiol-containing ligands) can move in the intracellular space not only by diffusion but also in an autowave mode. This hypothesis is based on the previously obtained data on autowave distribution of DNIC with glutathione following application of a drop of a solution of Fe2+ + glutathione onto the surface of a thin layer of a S-nitrosoglutathione solution. The appearance of autowaves is conditioned by a self-regulating self-sustained system arising in the process. This system consists of self-convertible DNIC and S-nitrosothiols as well as free ferrous iron ions, thiols and NO and can function in the autowave regime for several seconds with subsequent passage to a steady state maintained by chemical equilibrium between DNIC and their constituent components (free Fe2+ ions, thiols, S-nitrosothiols and NO). Possible advantages of autowave distribution of NO and its endogenous derivatives in the intracellular space over free diffusion, which might entail higher efficiency of their biological action, are discussed. 相似文献
104.
A F Vanin Iu I Vedernikov M E Galagan L N Kubrina Ia A Kuzmanis I Ia Kalvin'sh P I Mordvintsev 《Biokhimii?a (Moscow, Russia)》1990,55(8):1408-1413
Sodium trioxodinitrate (NaN2O3, Angeli salt) injected intraperitoneally to mice and intraarterially to rats is decomposed to form nitrogen oxide which is bound in tissues to the Fe2(+)-DETC complex or hemoglobin. The nitrosyl complexes formed thereby were studied by the EPR method. As can be judged from the number of complexes formed, nitrogen oxide produces 3-4% of exogenous NaN2O3 which provides for the hypotensive properties of Angeli salt as well as for its ability to cause the relaxation of isolated segments of rabbit femoral artery. In this respect NaN2O3 is by one order of magnitude less effective compared to sodium nitroprusside. 相似文献
105.
Exogenous dinitrosyl iron complexes (DNIC) with thiolate ligands as NO and NO+ donors are capable of exerting both regulatory and cytotoxic effects on diverse biological processes similarly to those characteristic of endogenous nitric oxide. Regulatory activity of DNIC (vasodilatory, hypotensive, suppressing thrombosis, increasing erythrocyte elasticity, accelerating skin wound healing, inducing penile erection, etc.) is determined by their capacity of NO and NO+ transfer to biological targets of the latter (heme- and thiol-containing proteins, respectively) due to higher affinity of the proteins for NO and NO+ than that of DNIC. Cytotoxic activity of DNIC is provided by rapid DNIC decomposition under action of iron-chelating compounds, resulting in appearance of NO and NO+ in cells and tissues in high amounts. The latter mechanism is suggested to cause the blocking effect of DNIC as cytotoxic effectors on the development of benign endometrial tumors in rats with experimental endometriosis. It is also proposed that a similar mechanism can operate to cause at least a delay of malignant tumor proliferation under action of DNIC. 相似文献
106.
Elaine A Dunlop Sara Seifan Tijs Claessens Christian Behrends Miriam AF Kamps Ewelina Rozycka Alain J Kemp Ravi K Nookala John Blenis Barry J Coull James T Murray Maurice AM van Steensel Simon Wilkinson Andrew R Tee 《Autophagy》2014,10(10):1749-1760
Birt-Hogg-Dubé (BHD) syndrome is a rare autosomal dominant condition caused by mutations in the FLCN gene and characterized by benign hair follicle tumors, pneumothorax, and renal cancer. Folliculin (FLCN), the protein product of the FLCN gene, is a poorly characterized tumor suppressor protein, currently linked to multiple cellular pathways. Autophagy maintains cellular homeostasis by removing damaged organelles and macromolecules. Although the autophagy kinase ULK1 drives autophagy, the underlying mechanisms are still being unraveled and few ULK1 substrates have been identified to date. Here, we identify that loss of FLCN moderately impairs basal autophagic flux, while re-expression of FLCN rescues autophagy. We reveal that the FLCN complex is regulated by ULK1 and elucidate 3 novel phosphorylation sites (Ser406, Ser537, and Ser542) within FLCN, which are induced by ULK1 overexpression. In addition, our findings demonstrate that FLCN interacts with a second integral component of the autophagy machinery, GABA(A) receptor-associated protein (GABARAP). The FLCN-GABARAP association is modulated by the presence of either folliculin-interacting protein (FNIP)-1 or FNIP2 and further regulated by ULK1. As observed by elevation of GABARAP, sequestome 1 (SQSTM1) and microtubule-associated protein 1 light chain 3 (MAP1LC3B) in chromophobe and clear cell tumors from a BHD patient, we found that autophagy is impaired in BHD-associated renal tumors. Consequently, this work reveals a novel facet of autophagy regulation by ULK1 and substantially contributes to our understanding of FLCN function by linking it directly to autophagy through GABARAP and ULK1. 相似文献
107.
108.
Timoshin AA Pisarenko OI Tskitishvili OV Serebriakova LI Studneva IM Drobotova DIu Ruuge EK Vanin AF 《Biofizika》2010,55(6):1099-1107
The injection of dinitrosyliron iron complexes with glutathione at the onset of 40-min rat regional myocardial ischemia was shown to exert a clear cardioprotective action by decreasing the infarct size and suppressing the cardiac rhythm disturbance. After the introduction of the preparation, its effective accumulation with protein thiol-containing ligands in the myocardial tissue was registered be the EPR method. It was also found that, as a result of postischemic reperfusion, the rate of the decrease in the content of these complexes in the ischemic area increases, which demonstrates the effective scavenging of short-lived reactive oxygen species by molecules of dinitrosyl iron complexes. 相似文献
109.
A. A. Timoshin O. I. Pisarenko O. V. Tskitishvili L. I. Serebryakova I. M. Studneva D. Yu. Drobotova E. K. Ruuge A. F. Vanin 《Biophysics》2010,55(6):999-1005
Injection of dinitrosyl iron complexes with glutathione at the onset of 40-min regional myocardial ischemia in rat was shown
to exert a clear cardioprotective action by decreasing the infarct size and suppressing the cardiac rhythm disturbance. After
introducing the preparation, its effective accumulation with protein thiol-containing ligands in the myocardial tissue was
registered be the EPR method. It was also found that in postischemic reperfusion, the rate of decrease in the content of these
complexes in the ischemic area increases, which reflects effective scavenging of short-lived reactive oxygen species by the
dinitrosyl iron complexes. 相似文献
110.
K. B. Shumaev I. V. Sviryaeva S. A. Gubkina T. S. Krivova A. F. Topunov A. F. Vanin E. K. Ruuge 《Biophysics》2010,55(3):406-411
It has been established that, in the presence of S-nitrosothiols, cysteine, and mitochondria, dinitrosyl iron complexes (DNIC)
coupled to low-molecular-weight ligands and proteins are formed. The concentration of DNIC depended on oxygen partial pressure.
It was shown that, under the conditions of hypoxia, the kinetics of the formation of low-molecular DNIC was biphasic. After
the replacement of anaerobic conditions of incubation with aerobic ones, the level of DNIC came down; in this case, protein
dinitrosyl complexes became more stable. We proposed that iron-and sulfur-containing proteins and low-molecular-weight iron
complexes are the sources of iron for DNIC formation in mitochondrial suspensions. It was shown that a combination of DNIC
and S-nitrosothiols inhibited effectively the respiration of cardiomyocytes. 相似文献